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Kobayashi, Yasuhiko; Funayama, Tomoo; Wada, Seiichi*; Taguchi, Mitsumasa; Watanabe, Hiroshi
Radiation Research, 161(1), p.90 - 91, 2004/01
A single cell irradiation system has been developed for targeting cells individually with a precise number of heavy ions to elucidate radiobiological effects of exactly one particle and to investigate the biological effects of low fluence irradiation with HZE particles. Using the heavy ion microbeam apparatus installed at JAERI-Takasaki, mammalian cells were irradiated in the atmosphere with a single or precise numbers of ions, 13.0 MeV/u 20Ne or 11.5 MeV/u 40Ar. The number of ions traversed the cells attached on the ion track detector CR-39 were counted with a plastic scintillator. Immediately after the irradiation, the position and the number of ion tracks traversed the cell was detected with etching of CR-39 from the opposite side of the cell with alkaline-ethanol solution at 37
C. The growths of the cells were observed individually up to 60 hours after irradiation.
Funayama, Tomoo; Wada, Seiichi; Kobayashi, Yasuhiko
Radiation Research, 161(1), p.111 - 112, 2004/01
Using the heavy ion microbeam apparatus, mammalian cells were irradiated in the atmosphere with a single or precise numbers of 
Ar heavy ions (11.5 MeV/u) with a spatial resolution of a few microns. Positional data of the individual cells attached on the ion track detector CR-39 were obtained at the off-line microscope before irradiation, then the targeting and irradiation of the cells were performed semi-automatically at the on-line microscope of the microbeam apparatus according to the obtained data. Immediately after irradiation, the position and the number of ion tracks traversed the cell was detected with etching of CR-39 at 37 C. The growths of the cells were observed individually up to 60 hours after irradiation. The continuous observation of the individual cell growth indicated that single Ar ion traversal of cell nucleolus resulted to complete growth inhibition of the irradiated cells.